LAN Yan, CUI Baoshan, HAN Zhen, LI Xia, LI Fengju, ZHANG Yongtao. Spatial Distribution and Environmental Determinants of Denitrification Enzyme Activity in Reed-Dominated Raised Fields[J]. Chinese Geographical Science, 2015, 25(4): 438-450. doi: 10.1007/s11769-014-0721-2
Citation: LAN Yan, CUI Baoshan, HAN Zhen, LI Xia, LI Fengju, ZHANG Yongtao. Spatial Distribution and Environmental Determinants of Denitrification Enzyme Activity in Reed-Dominated Raised Fields[J]. Chinese Geographical Science, 2015, 25(4): 438-450. doi: 10.1007/s11769-014-0721-2

Spatial Distribution and Environmental Determinants of Denitrification Enzyme Activity in Reed-Dominated Raised Fields

doi: 10.1007/s11769-014-0721-2
Funds:  Under the auspices of National Science Fund for Distinguished Young Scholars (No. 51125035), National Science Foundation for Innovative Research Group (No. 51121003), Major Science and Technology Program for Water Pollution Control and Treatment (No. 2009ZX07209-008)
More Information
  • Corresponding author: CUI Baoshan. E-mail: cuibs@bnu.edu.cn
  • Received Date: 2013-05-09
  • Rev Recd Date: 2013-09-09
  • Publish Date: 2015-04-27
  • Denitrification is an important process of nitrogen removal in lake ecosystems. However, the importance of denitrification across the entire soil-depth gradients including subsurface layers remains poorly understood. This study aims to determine the spatial pattern of soil denitrification enzyme activity (DEA) and its environmental determinants across the entire soil depth gradients in the raised fields in Baiyang Lake, North China. In two different zones of the raised fields (i.e., water boundary vs. main body of the raised fields), the soil samples from -1.0 m to 1.1 m depth were collected, and the DEA and following environmental determinants were quantified: soil moisture, pH, total nitrogen (TN), ammonia nitrogen (NH4+-N), nitrate nitrogen (NO3--N), total organic carbon (TOC), and rhizome biomass of Phragmites australis. The results showed that the soil DEA and environmental factors had a striking zonal distribution across the entire soil depth gradients. The soil DEA reached two peak values in the upper and middle soil layers, indicating that denitrification are important in both topsoil and subsurface of the raised fields. The correlation analysis showed that the DEA is negatively correlated with the soil depth (p < 0.05). However, this phenomenon did not occur in the distance to the water edge, except in the upper layers (from 0.2 m to 0.7 m) of the boundary zone of the raised fields. In the main body of the raised fields, the DEA level remained high; however, it showed no significant relationship with the distance to the water edge. The linear regression analysis showed significant positive correlation of the DEA with the soil TN, NO3--N, NH4+-N, and TOC; whereas it showed negative correlation with soil pH. No significant correlations with soil moisture and temperature were observed. A positive correlation was also found between the DEA and rhizome biomass of P. australis.
  • [1] Akatsuka T, Mitamura O, 2011. Response of denitrification rate associated with wetting and drying cycles in a littoral wetland area of Lake Biwa, Japan. Limnology, 12(2): 127-135. doi:  10.1007/s10201-010-0329-x
    [2] Al Ghadban A N, Uddin S, Maltby E et al., 2012. Denitrification potential of the Northern Arabian Gulf—An experimental study. Environmental Monitoring and Assessment, 184(12): 7103-7112. doi:  10.1007/s10661-011-2483-y
    [3] Arce M I, Gómez R, Suárez M L et al., 2013. Denitrification rates and controlling factors in two agriculturally influenced temporary Mediterranean saline streams. Hydrobiologia, 700(1): 169-185. doi:  10.1007/s10750-012-1228-4
    [4] Bai J, Ouyang H, Deng W et al., 2005. Spatial distribution characteristics of organic matter and total nitrogen of marsh soils in river marginal wetlands. Geoderma, 124(1-2): 181-192. doi:  10.1016/j.geoderma.2004.04.012
    [5] Bai J, Wang J, Yan D et al., 2012. Spatial and temporal distributions of soil organic carbon and total nitrogen in two marsh wetlands with different flooding frequencies of the Yellow River Delta, China. Clean-Soil, Air, Water, 40(10): 1137-1144. doi:  10.1002/clen.201200059
    [6] Bandy M S, 2005. Energetic efficiency and political expediency in Titicaca Basin raised field agriculture. Journal of Anthropological Archaeology, 24(3): 271-296. doi: 10.1016/j.jaa. 2005.03.002
    [7] Bastviken S K, Eriksson P G, Premrov A et al., 2005. Potential denitrification in wetland sediments with different plant species detritus. Ecological Engineering, 25(2): 183-190. doi:  10.1016/j.ecoleng.2005.04.013
    [8] Batson J A, Mander U, Mitsch W J, 2012. Denitrification and a nitrogen budget of created riparian wetlands. Journal of Environmental Quality, 41(6): 2024-2032. doi: 10.2134/jeq2011. 0449
    [9] Burford R A, Bremner J M, 1975. Relationships between the denitrification capacities of soils and total, water-soluble and readily decomposable soil organic matter. Soil Biology and Biochemistry, 7(6): 389-394. doi: 10.1016/0038-0717(75) 90055-3
    [10] Burgin A J, Groffman P M, Lewis D N, 2010. Factors regulating denitrification in a riparian wetland. Soil Science Society of America Journal, 74(5): 1826-1833. doi: 10.2136/sssaj2009. 0463
    [11] Caffrey J M, Kemp W M, 1991. Seasonal and spatial patterns of oxygen production, respiration and root-rhizome release in Potamogeton perfoliatus L. and Zostera marina L. Aquatic Botany, 40(2): 109-128. doi:  10.1016/0304-3770(91)90090-R
    [12] Cao Y, Green P G, Holden P A, 2008. Microbial community composition and denitrifying enzyme activities in salt marsh sediments. Applied and Environmental Microbiology, 74(24): 7585-7595. doi:  10.1128/AEM.01221-08
    [13] Carney H J, Binford M W, Kolata A L et al., 1993. Nutrient and sediment retantion in Andean raised-field agriculture. Nature, 364: 131-133. doi:  10.1038/364131a0
    [14] CCLCAC (Compilation Committee of Local Chronicles of Anxin County), 2000. Anxin County Annals. Beijing: Xinhua Pulishing House, 219. (in Chinese)
    [15] Cui B, Li X, Zhang K, 2010. Classification of hydrological conditions to assess water allocation schemes for Lake Baiyangdian in North China. Journal of Hydrology, 385(1-4): 247-256. doi:  10.1016/j.jhydrol.2010.02.026
    [16] Denevan W M, 1970. Aboriginal drained-field cultivation in the Americas. Science, 169(3946): 647-654. doi: 10.1126/science. 169.3946.647
    [17] Dhondt K, Boeckx P, Hofman G et al., 2004. Temporal and spatial patterns of denitrification enzyme activity and nitrous oxide fluxes in three adjacent vegetated riparian buffer zones. Biology and Fertility of Soils, 40(4): 243-251. doi: 10.1007/ s00374-004-0773-z
    [18] Erickson C L, Candler K L, 1989. Raised fields and sustainable agriculture in the Lake Titicaca basin of Peru. In: Browder J O et al. (eds.). Fragile Lands of Latin America. Boulder: Westview Press, 230-243.
    [19] García-Ruiz R, Pattinson S N, Whitton B A, 1998. Denitrification in river sediments: Relationship between process rate and properties of water and sediment. Freshwater Biology, 39(3): 467-476. doi:  10.1046/j.1365-2427.1998.00295.x
    [20] Ge Y, Zhang C, Jiang Y et al., 2011. Soil microbial abundances and enzyme activities in different rhizospheres in an integrated vertical flow constructed wetland. Clean-Soil, Air, Water, 39(3): 206-211. doi:  10.1002/clen.201000230
    [21] Geisseler D, Horwath W R, 2009. Relationship between carbon and nitrogen availability and extracellular enzyme activities in soil. Pedobiologia, 53(1): 87-98. doi: 10.1016/j.pedobi. 2009.06.002
    [22] Goulder R, 1990. Extracellular enzyme-activities associated with epiphytic microbiota on submerged stems of the reed Phragmites australis. Fems Microbiology and Ecology, 73(4): 323-330. doi:  10.1111/j.1574-6968.1990.tb03956.x
    [23] Griffiths R P, Homann P S, Riley R, 1998. Denitrification enzyme activity of Douglas-fir and red alder forest soils of the Pacific Northwest. Soil Biology and Biochemistry, 30(8-9): 1147-1157. doi:  10.1016/S0038-0717(97)00185-5
    [24] Hefting M, Clement J C, Dowrick D et al., 2004. Water table elevation controls on soil nitrogen cycling in riparian wetlands along a European climatic gradient. Biogeochemistry, 67(1): 113-134. doi:  10.1023/B:BIOG.0000015320.69868.33
    [25] Helyar K R, Cregan P D, Godyn D L, 1990. Soil acidity in new-south-wales-current pH values and estimates of acidification rates. Australian Journal of Soil Research, 28(4): 523-537. doi:  10.1071/SR9900523
    [26] Hill A R, Devito K J, Campagnolo S et al., 2000. Subsurface denitrification in a forest riparian zone: Interactions between hydrology and supplies of nitrate and organic carbon. Biogeochemistry, 51(2): 193-223. doi:  10.1023/A:1006476514038
    [27] Howarth R W, Billen G, Swaney D et al., 1996. Regional nitrogen budgets and riverine N and P fluxes for the drainages to the North Atlantic Ocean: Natural and human influences. Biogeochemistry, 35(1): 75-139. doi:  10.1007/978-94-009-1776-7_3
    [28] Jolivet C, Arrouays D, Lévèque J et al., 2003. Organic carbon dynamics in soil particle-size separates of sandy Spodosols when forest is cleared for maize cropping. European Journal of Soil Science, 54(2): 257-268. doi: 10.1046/j.1365-2389. 2003.00541.x
    [29] Karunaratne S, Asaeda T, Yutani K, 2004. Age-specific seasonal storage dynamics of Phragmites australis rhizomes: A preliminary study. Wetlands Ecology and Management, 12(5): 343-351. doi:  10.1007/s11273-004-6245-2
    [30] Khalil M I, Richards K G, 2011. Denitrification enzyme activity and potential of subsoils under grazed grasslands assayed by membrane inlet mass spectrometer. Soil Biology and Biochemistry, 43(9): 1787-1797. doi: 10.1016/j.soilbio.2010.08. 024
    [31] Li F, Zhang Q, Tang C et al., 2011. Denitrifying bacteria and hydrogeochemistry in a natural wetland adjacent to farmlands in Chiba, Japan. Hydrological Processes, 25(14): 2237-2245. doi: 10.1002/hyp. 7988
    [32] Li X, Cui B, Yang Q et al., 2012. Detritus quality controls macrophyte decomposition under different nutrient concentrations in a eutrophic shallow lake, North China. PLOS ONE, 7(7): e42042. doi:  10.1371/journal.pone.0042042
    [33] Liu W, Liu G, Zhang Q, 2011. Influence of vegetation characteristics on soil denitrification in shoreline wetlands of the Danjiangkou Reservoir in China. Clean-Soil, Air, Water, 39(2): 109-115. doi:  10.1002/clen.200900212
    [34] Mitsch W J, 1992. Landscape design and the role of created, restored, and natural riparian wetlands in controlling nonpoint source pollution. Ecological Engineering, 1(1-2): 27-47. doi:  10.1016/0925-8574(92)90024-V
    [35] Nye P H, 1981. Changes of pH across the rhizosphere induced by roots. Plant and Soil, 61(1-2): 7-26. doi:  10.1007/BF02277359
    [36] Pan Y, Ye L, Ni B et al., 2012. Effect of pH on N2O reduction and accumulation during denitrification by methanol utilizing denitrifiers. Water Research, 46(15): 4832-4840. doi: 10.1016/ j.watres.2012.06.003
    [37] Peterson M E, Curtin D, Thomas S et al., 2013. Denitrification in vadose zone material amended with dissolved organic matter from topsoil and subsoil. Soil Biology & Biochemistry, 61: 96-104. doi:  10.1016/j.soilbio.2013.02.010
    [38] Priha O, Hallantie T, Smolander A, 1999. Comparing microbial biomass, denitrification enzyme activity, and numbers of nitrifiers in the rhizospheres of Pinus sylvestris, Picea abies and Betula pendula seedlings by microscale methods. Biology and Fertility of Soils, 30(1-2): 14-19. doi:  10.1007/s003740050581
    [39] Racchetti E, Bartoli M, Soana E et al., 2011. Influence of hydrological connectivity of riverine wetlands on nitrogen removal via denitrification. Biogeochemistry, 103(1-3): 335-354. doi:  10.1007/s10533-010-9477-7
    [40] Rea N, 1996. Water levels and Pharagmites: Decline from lack of regeneration or dieback from shoot death. Folia Geobotanica and Phytotaxonomica, 31(1): 85-90. doi:  10.1007/BF02803997
    [41] Reisinger A J, Blair J M, Rice C W et al., 2013. Woody vegetation removal stimulates riparian and benthic denitrification in tallgrass prairie. Ecosystems, 16(4): 547-560. doi: 10.1007/ s10021-012-9630-3
    [42] Rhee D S, Woo H, Kwon B A et al., 2008. Hydraulic resistance of some selected vegetation in open channel flows. River Research and Applications, 24(5): 673-687. doi: 10.1002/ rra.1143
    [43] Risser P G, 1990. The ecological importance of land-water ecotones. In: Naiman R J et al. (eds.). The Ecology and Management of Aquatic-Terrestrial Ecotones. Lancs: Taylor & Francis Group, 7-18.
    [44] Smith M S, Tiedje J M, 1979. Phases of denitrification following oxygen depletion in soil. Soil Biology and Biochemistry, 11(3): 261-267. doi:  10.1016/0038-0717(79)90071-3
    [45] Song K, Lee S H, Kang H, 2011. Denitrification rates and community structure of denitrifying bacteria in newly constructed wetland. European Journal of Soil Biology, 47(1): 24-29. doi:  10.1016/j.ejsobi.2010.10.003
    [46] Stevenson B A, Schipper L A, McGill A et al., 2011. Denitrification and availability of carbon and nitrogen in a well-drained pasture soil amended with particulate organic carbon. Journal of Environmental Quality, 40(3): 923-930. doi: 10.2134/jeq 2010.0463
    [47] Turner B L, 1974. Prehistoric intensive agriculture in the Mayan lowlands. Science, 185(4146): 118-124. doi: 10.1126/science. 185.4146.118
    [48] Wang L, Yin C, Wang W et al., 2010. Phosphatase activity along soil C and P gradients in a reed-dominated wetland of North China. Wetlands, 30(3): 649-655. doi: 10.1007/s13157-010- 0055-5
    [49] Wang W, Yin C, 2008. The boundary filtration effect of reed-dominated ecotones under water level fluctions. Wetlands Ecology and Management, 16(1): 65-76. doi: 10.1007/s11273- 007-9057-3
    [50] White S D, Deegan B M, Ganf G G, 2007. The influence of water level fluctuation on the potential for convective flow in the emergent macrophytes Typha domingensis and Phragmites australis. Aquatic Botany, 86(4): 369-376. doi: 10.1016/j. aquabot. 2007.01.006
    [51] Wilson C, Simpson I A, Currie E J, 2002. Soil management in pre-Hispanic raised field systems: Micromorphological evidence from Hacienda Zuleta, Ecuador. Geoarchaeology: An International Journal, 17(3): 261-283. doi:  10.1002/gea.10015
    [52] Xu F, Yang Z F, Chen B et al., 2011. Ecosystem health assessment of the plant-dominated Baiyangdian Lake based on eco-exergy. Ecological Modelling, 222(1): 201-209. doi:  10.1016/j.ecolmodel.2010.09.027
    [53] Yu H, Song Y, Xi B et al., 2012. Denitrification potential and its correlation to physico-chemical and biological characteristics of saline wetland soils in semi-arid regions. Chemosphere, 89(11): 1339-1346. doi:  10.1016/j.chemosphere.2012.05.088
    [54] Zhu G, Wang S, Wang W et al., 2013. Hotspots of anaerobic ammonium oxidation at land-freshwater interfaces. Nature Geoscience, 6: 103-107. doi:  10.1038/ngeo1683
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Spatial Distribution and Environmental Determinants of Denitrification Enzyme Activity in Reed-Dominated Raised Fields

doi: 10.1007/s11769-014-0721-2
Funds:  Under the auspices of National Science Fund for Distinguished Young Scholars (No. 51125035), National Science Foundation for Innovative Research Group (No. 51121003), Major Science and Technology Program for Water Pollution Control and Treatment (No. 2009ZX07209-008)
    Corresponding author: CUI Baoshan. E-mail: cuibs@bnu.edu.cn

Abstract: Denitrification is an important process of nitrogen removal in lake ecosystems. However, the importance of denitrification across the entire soil-depth gradients including subsurface layers remains poorly understood. This study aims to determine the spatial pattern of soil denitrification enzyme activity (DEA) and its environmental determinants across the entire soil depth gradients in the raised fields in Baiyang Lake, North China. In two different zones of the raised fields (i.e., water boundary vs. main body of the raised fields), the soil samples from -1.0 m to 1.1 m depth were collected, and the DEA and following environmental determinants were quantified: soil moisture, pH, total nitrogen (TN), ammonia nitrogen (NH4+-N), nitrate nitrogen (NO3--N), total organic carbon (TOC), and rhizome biomass of Phragmites australis. The results showed that the soil DEA and environmental factors had a striking zonal distribution across the entire soil depth gradients. The soil DEA reached two peak values in the upper and middle soil layers, indicating that denitrification are important in both topsoil and subsurface of the raised fields. The correlation analysis showed that the DEA is negatively correlated with the soil depth (p < 0.05). However, this phenomenon did not occur in the distance to the water edge, except in the upper layers (from 0.2 m to 0.7 m) of the boundary zone of the raised fields. In the main body of the raised fields, the DEA level remained high; however, it showed no significant relationship with the distance to the water edge. The linear regression analysis showed significant positive correlation of the DEA with the soil TN, NO3--N, NH4+-N, and TOC; whereas it showed negative correlation with soil pH. No significant correlations with soil moisture and temperature were observed. A positive correlation was also found between the DEA and rhizome biomass of P. australis.

LAN Yan, CUI Baoshan, HAN Zhen, LI Xia, LI Fengju, ZHANG Yongtao. Spatial Distribution and Environmental Determinants of Denitrification Enzyme Activity in Reed-Dominated Raised Fields[J]. Chinese Geographical Science, 2015, 25(4): 438-450. doi: 10.1007/s11769-014-0721-2
Citation: LAN Yan, CUI Baoshan, HAN Zhen, LI Xia, LI Fengju, ZHANG Yongtao. Spatial Distribution and Environmental Determinants of Denitrification Enzyme Activity in Reed-Dominated Raised Fields[J]. Chinese Geographical Science, 2015, 25(4): 438-450. doi: 10.1007/s11769-014-0721-2
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